gga-miR-155对MDCC-MSB1细胞生物学行为的影响

doi:10.11843/j.issn.0366-6964.2018.11.022

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (11): 2496-2504.doi: 10.11843/j.issn.0366-6964.2018.11.022

gga-miR-155对MDCC-MSB1细胞生物学行为的影响

余祖华¹, 丁轲^1*, 郁川¹, 贾艳艳¹, 何雷¹, 廖成水¹, 李静¹, 张春杰¹, 李银聚¹, 吴庭才¹, 程相朝^1,2, 张梦珂¹, 邱静静¹, 罗俊^1,3

1. 河南科技大学动物疫病与公共卫生重点实验室, 洛阳市活载体生物材料与动物疫病防控重点实验室, 洛阳 471023;
2. 洛阳职业技术学院, 洛阳 471003;
3. 河南省农业科学院动物免疫学重点实验室, 农业部动物免疫学重点实验室, 河南省动物免疫学重点实验室, 郑州 450002

收稿日期:2018-03-05 出版日期:2018-11-23 发布日期:2018-11-23
通讯作者: 丁轲,E-mail:keding19@163.com
作者简介:余祖华(1977-),女,河南商城人,副教授,博士,主要从事动物分子免疫学研究,E-mail:yzhd05@163.com
基金资助:
国家自然科学基金（U1504308；31702207）；河南科技大学博士科研启动基金项目（13480068）；河南科技大学省部级科技创新平台培育项目（2015SPT004）

Effect of gga-miR-155 on the Biological Behavior of MDCC-MSB1 Cells

YU Zu-hua¹, DING Ke^1*, YU Chuan¹, JIA Yan-yan¹, HE Lei¹, LIAO Cheng-shui¹, LI Jing¹, ZHANG Chun-jie¹, LI Yin-ju¹, WU Ting-cai¹, CHENG Xiang-chao^1,2, ZHANG Meng-ke¹, QIU Jing-jing¹, LUO Jun^1,3

1. Key Lab of Animal Disease and Public Health, Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, Luoyang 471023, China;
2. Luoyang Polytechnic, Luoyang 471003, China;
3. Key Laboratory of Animal Immunology of Henan Academy of Agricultural Sciences, Key Laboratory of Animal Immunology of Ministry of Agriculture, Key Laboratory of Animal Immunology in Henan Province, Zhengzhou 450002, China

Received:2018-03-05 Online:2018-11-23 Published:2018-11-23

摘要/Abstract

摘要：

为了解gga-miR-155对MDCC-MSB1细胞生物学行为的影响，将人工合成的gga-miR-155模拟物、gga-miR-155抑制物以及阴性对照瞬时转染MDCC-MSB1细胞。然后采用实时荧光定量PCR （RT-qPCR）检测MDCC-MSB1细胞中gga-miR-155的表达水平，CCK-8法检测转染后细胞增殖能力的变化，流式细胞术检测MDCC-MSB1细胞的细胞周期与凋亡的变化，Transwell检测MDCC-MSB1细胞的迁移、侵袭能力的变化。结果显示，gga-miR-155模拟物可显著上调MDCC-MSB1细胞gga-miR-155表达水平，促进MDCC-MSB1细胞增殖，细胞G1期细胞减少，S和G2期细胞增加，同时凋亡减少，迁移、侵袭能力增加；gga-miR-155抑制物可显著下调MDCC-MSB1细胞gga-miR-155表达水平，抑制MDCC-MSB1细胞增殖，细胞G1期细胞增加、S和G2期细胞减少，同时凋亡增加，迁移、侵袭能力下降。结果表明，gga-miR-155可促进MDCC-MSB1细胞增殖、迁移和侵袭，抑制其凋亡。

Abstract:

In order to detect the effect of gga-miR-155 on the biological behavior of MDCC-MSB1 cells, MDCC-MSB1 cells were transiently transfected with gga-miR-155 mimic, gga-miR-155 inhibitor, and negative control which were artificial synthesized. Then, the expression of gga-miR-155 was detected by real-time PCR (qRT-PCR); the cell proliferation was detected by CCK-8 assay; the cell cycle and apoptosis were analyzed by flow cytometry; and the cell migration and invasive ability were determined by transwell assay. Results showed that gga-miR-155 mimic up-regulated the expression level of gga-miR-155 in MDCC-MSB1 cells. After gga-miR-155 mimic transfection, the proliferation of MDCC-MSB1 cells was promoted, G1 phase cells were decreased, S and G2 cells were increased, the apoptosis rate was reduced,the migration invasion capability were increased in gga-miR-155 mimic transfected cells. On the contrary, the gga-miR-155 inhibitor significantly down-regulated the expression level of gga-miR-155 in MDCC-MSB1 cells. When the gga-miR-155 inhibitor was transfected into MDCC-MSB1 cells, cell proliferation was inhibited,G1 phase cells were increased, S and G2 cells were reduced, the cell apoptosis was increased, and the migration invasion capability were suppressed. The results showed that gga-miR-155 could promote the proliferation, migration and invasion of MDCC-MSB1 cells and inhibit their apoptosis.

中图分类号:

余祖华, 丁轲, 郁川, 贾艳艳, 何雷, 廖成水, 李静, 张春杰, 李银聚, 吴庭才, 程相朝, 张梦珂, 邱静静, 罗俊. gga-miR-155对MDCC-MSB1细胞生物学行为的影响[J]. 畜牧兽医学报, 2018, 49(11): 2496-2504.

YU Zu-hua, DING Ke, YU Chuan, JIA Yan-yan, HE Lei, LIAO Cheng-shui, LI Jing, ZHANG Chun-jie, LI Yin-ju, WU Ting-cai, CHENG Xiang-chao, ZHANG Meng-ke, QIU Jing-jing, LUO Jun. Effect of gga-miR-155 on the Biological Behavior of MDCC-MSB1 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2496-2504.

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gga-miR-155对MDCC-MSB1细胞生物学行为的影响

Effect of gga-miR-155 on the Biological Behavior of MDCC-MSB1 Cells

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